Flow Cytometry Basic Principles And Applications
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Flow Cytometry
Author: Alice Longobardi Givan
language: en
Publisher: John Wiley & Sons
Release Date: 2013-04-10
Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.
Cellular Diagnostics
Author: Ulrich Sack
language: en
Publisher: Karger Medical and Scientific Publishers
Release Date: 2009-01-01
This book is the updated English version of the 2006 German bestseller Zellulare Diagnostik, a comprehensive presentation of flow cytometry and its applications. While some techniques of immunophenotyping by flow cytometry already are routine procedures in the laboratory, new methods for the functional characterization of cells, the analysis of rare cells, and the diagnosis of complex materials have only begun to win wide recognition. New approaches such as slide-based cytometry will lead to an increase in the use of cytometric techniques. Multiparameter approaches will further improve analysis. The book provides a comprehensive and detailed compilation of all aspects of flow cytometry in research and the clinic. For newcomers it offers a thorough introduction, for advanced users, specific protocols and interpretation assistance.
Clinical Flow Cytometry
This book is a focused review of clinical flow cytometry, and is meant to be helpful in daily clinical practice for those just beginning to learn flow cytometry as well as those with years of experience. Covering the basic principles of flow cytometry, and then engaging in detailed reviews of the flow cytometric evaluation of B-cells, plasma cells, T-cells, and myeloid cells, it packs a wide variety of immunophenotypic data into one volume. Moreover, this book covers both normal and abnormal findings for each lineage, and highlights key pitfalls to avoid making diagnostic mistakes. Many of the most common neoplastic entities are reviewed, and signature findings are highlighted. Using the updated nomenclature for clinical hematologic malignancies provided by the revised 4th edition of the WHO classification system, the book is current in its approach and content. Whenever possible, detailed colored examples of flow cytometric plots are provided to help convey the important diagnostic findings. Most importantly, a review of current applications of flow cytometry in minimal residual disease is provided to assist in both the development and interpretation of these assays. Written by experts in the field, the result is a practical resource for use as an everyday clinical reference.