Breaking Through Biology S Data Wall Expanding The Known Tree Of Life By Over 10x Using A Global Biodiscovery Pipeline
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Gene Expression Analysis
Author: Nalini Raghavachari
language: en
Publisher: Springer Nature
Release Date: 2025-02-03
This second edition volume expands on the previous edition with updates on the latest methodologies in the transcriptomics field. The chapters in this book cover topics such as spatial omics, long-read sequencing technology, tissue microarrays, analysis of saliva and extracellular vesicles, machine learning and artificial intelligence-based approaches for analysis of singe cells transcriptome, and large sets of data on multi-omics including transcriptomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Gene Expression Analysis: Methods and Protocols, Second Edition is a valuable resource for advanced undergraduate and graduate students studying gene expression analysis, and scientists interested in learning more about this rapidly advancing field.
Fluorescence In Situ Hybridization (FISH) - Application Guide
Author: Thomas Liehr
language: en
Publisher: Springer Science & Business Media
Release Date: 2008-11-26
This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.
Structural Proteomics
The objective of this volume is to provide readers with a current view of all aspects of the 'pipeline' that takes protein targets to structures and how these have been optimised. This volume includes chapters describing, in-depth, the individual steps in the Structural Genomics pipeline, as well as less detailed overviews of individual Structural Genomics initiatives. It is the first book of protocols to cover techniques in a new and emerging field.