Advances In Gapdh Protein Analysis A Functional And Biochemical Approach
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Advances in GAPDH Protein Analysis: A Functional and Biochemical Approach
Author: Shanmugasundaram Ganapathy-Kanniappan
language: en
Publisher: Springer
Release Date: 2018-01-16
This book presents modern and classic analytical approaches that are crucial for the biochemical and functional characterization of the archetypal protein, glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The distinguishing feature of the book is that it covers, in addition to other methods, some of the uncommon but valuable techniques as well. For example, in-gel visualization of enzyme activity, immunoblotting protocols for native (non-denatured) proteins, and proteins resolved by pH-gradient [IEF-isoelectrofocusing], etc. These expedient methods are relevant and vital for the verification of biochemical properties of GAPDH, or similar protein of interest. This work outlines detailed protocols that are essential to investigate classical (cellular) and recently reported extracellular (secretory) isoforms of GAPDH. Precisely, the book covers techniques pertinent to enzymatic and non-enzymatic analysis of GAPDH that include, but not limited to, electrophoretic mobility shift assay (EMSA), two-dimensional (2D)-immunoblotting, immunofluorescence/confocal microscopy, mass spectrometry, ion-exchange and affinity chromatography. Readers will discover the importance of the experimental methods described in the book as they relate to the evaluation of the role and significance of GAPDH. Furthermore, majority of the methods described in the book have also been validated in the author’s laboratory, besides other research groups worldwide, underlining the repeatability and reproducibility of the protocols. Each method begins with an abstract and a brief background emphasizing its application and relevance. This will enable the readers to determine the choice of experimental design according to their research objectives. The book explains the methods systematically with ample illustrations to facilitate quick and easy comprehension of the practical knowledge. Although the book is focused on GAPDH, many of the protocols may be adopted to other proteins or enzymes with minimal modifications. Noteworthy, it is unequivocally established that GAPDH is a multifunctional protein involved in several cellular processes of health & disease conditions. Hence, this book will be a valuable practical guide for young researchers, scientists and clinician-scientists.
Luminescent Proteins—Advances in Research and Application: 2013 Edition
Luminescent Proteins—Advances in Research and Application: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Luciferases. The editors have built Luminescent Proteins—Advances in Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Luciferases in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Luminescent Proteins—Advances in Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Genetic advancements for improving the plant tolerance to biotic and abiotic stresses
Author: Krishnanand P. Kulkarni
language: en
Publisher: Frontiers Media SA
Release Date: 2024-05-31
Crop plants are constantly exposed to multiple abiotic (such as drought, salinity, cold, flooding, heavy metal, and heat) and/or biotic (bacterial/fungal/viral) stress factors that hinder their growth and development, subsequently leading to decreases in quality and yield. During the last two decades, many classical genetic and breeding approaches have been used to develop stress-tolerant and climate-adaptable plants that can provide a better yield to meet food demands. Climate change poses a major risk to food security as the world faces frequent floods, droughts, heat waves, and the emergence of new invasive pests and diseases. Novel genomic and genetic approaches look promising to improve plant resilience under stress conditions and achieve sustainable crop improvements. Recent advances in sequencing technologies have facilitated the generation of a plethora of genomic resources in a variety of crop and plant species. With the increased availability of genomic and transcriptomic data, an increasing number of quantitative trait loci and candidate genes are being identified for their application in improving plant tolerance to abiotic and biotic stresses. New approaches such as genomic selection and genomic-assisted breeding have been utilized to develop stress-tolerant cultivars in a variety of plant species. Furthermore, transgenics and rapidly evolving CRISPR technology offer great potential for plant improvement. This Research Topic aims to provide insights into the molecular and genetic factors involved in imparting abiotic and biotic stress tolerance in plants and their application in enhancing plant adaptation to these stress conditions. To review the progress in this research category, we invite manuscripts related to the plant responses to abiotic/biotic stresses and trait improvement through genomic selection, and transgenic or gene-editing approaches. Studies including physiological, biochemical, and molecular genetic analyses revealing the mechanisms involved in plant response to abiotic/biotic stresses are welcome. Topic editor Dr. Balaji Aravindhan Pandian is employed by Enko Chem Inc. All other Topic Editors declare no competing interests with regard to the Research Topic subject.