Advanced Biophysical Techniques In Biosciences
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Biophysical Techniques in Biosciences
This book details the latest advancements in spectroscopic, analytical and imaging techniques, emphasizing their crucial roles in both research and biomedical diagnostics. The initial chapters introduce the fundamental principles of the techniques, highlighting the use of optical spectroscopies for disease diagnosis, such as oral cancer. The book also explores their innovative applications, such as quantitative optical phase imaging, and the examination of biopolymers like starch through spectroscopy and microscopy. Further, the book discusses cutting-edge developments in biomaterials essential for understanding tissue engineering and the innovative use of synthesized bioactive glasses. The chapters also examine revolutionary methods such as HPLC and HPTLC techniques for detailed analysis at unprecedented scales and for observing various processes in health and disease. Importantly, the book reviews the impact of machine learning in enhancing the accuracy of disease diagnoses through nonlinear optical microscopy. The book also presents technological breakthroughs in the transformative impact of these techniques in developing diagnostic and therapeutic solutions. This book is intended for students, researchers, and professionals in biophysics, medical imaging, and biomedical engineering. Key Features: Highlights innovative applications such as quantitative optical phase imaging and the use of spectroscopy in disease diagnosis Explores the fundamental principles of advanced spectroscopic and imaging techniques Demonstrates the role of new technologies like synthesized biomaterials and applications of HPLC techniques Discusses the integration of machine learning with nonlinear optical microscopy to enhance the accuracy of disease diagnoses Presents the latest developments in biomaterials that are revolutionizing tissue engineering
Advanced Techniques in Biophysics
Author: José Luis R. Arrondo
language: en
Publisher: Springer Science & Business Media
Release Date: 2007-04-21
Technical advancements are basic elements in our life. In biophysical studies, new applications and improvements in well-established techniques are being implemented every day. This book deals with advancements produced not only from a technical point of view, but also from new approaches that are being taken in the study of biophysical samples, such as nanotechniques or single-cell measurements. This book constitutes a privileged observatory for reviewing novel applications of biophysical techniques that can help the reader enter an area where the technology is progressing quickly and where a comprehensive explanation is not always to be found.
Advanced Biophysical Techniques in Biosciences
This book presents an overview of advanced biophysical techniques that can be used to understand the physicochemical properties of biomolecules and biomaterials and expand their potential for biomedical applications. It is split into two parts, the first covering advanced biophysical techniques and the second covering bioscience applications. Adequate knowledge about the behavior of biomacromolecules is essential for standardizing their applications in various industries. These properties are strongly influenced by the composition, chain structure (e.g. linear or branch), linkage patterns, and molecular weight of the biomolecules. This book describes the various internal and external factors that develop the structural and functional properties of biomolecules. Further, it covers the advanced techniques that can be used to discover and enhance these properties, such as scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray crystallography, fluorescence spectroscopy, surface plasmon resonance, surface-enhanced Raman spectroscopy, force spectroscopy, optical tweezers, and more. It also covers high-performance liquid chromatography for biomolecule detection, including sample preparation, column selection, mobile phase determination, and the choice of an appropriate detector for the investigation. This book serves as a modern resource on the topic, providing an in-depth analysis of various important physicochemical properties as well as their wide range of applications, including in pharmaceuticals, bioimaging/sensing, cancer therapy, food sciences, textiles, scaffolds, drug delivery, and tissue engineering. Readers are presented with several invasive and non-invasive techniques that can be used for the characterization of biomacromolecules along with many types of physical, chemical, and physicochemical modifications that can be used to enhance their usage.