Techniques In Quantification And Localization Of Gene Expression
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Techniques in Quantification and Localization of Gene Expression
Author: Bruce K. Patterson
language: en
Publisher: Springer Science & Business Media
Release Date: 2012-12-06
Can the son or daughter of a baseball pitcher or cricket bowler throw a ball 100 miles an hour? Is the son or daughter of an opera singer also an opera singer? Is a house with functional light switches lit? The line of thinking in these rhetorical questions also applies to human genetics. What do baseball pitchers, opera sing ers, light switches, and the Human Genome Project have in common? These questions address the issue of potential versus realization of function. Although sons and daughters of baseball pitchers and opera singers may have inherited the mechanical attributes to be baseball pitchers and opera singers, they may not, at any point in time, be baseball pitchers or opera singers. A house with functional light switches is not lit unless the light switches are on. Similarly, all of the genes discovered and sequenced as a result of the Human Genome Project are not expressed at the same time. Genome project information will allow us to deter mine the repertoire of genes in an individual, which is analogous to determining where the light switches in a house are located and whether they are functional (a mutation or deletion in the Genome Project Model). The pattern of "on" light switches in a house gives us functional information as to what the family inside is doing (e. g. , eating, reading, sleeping). Similarly, the pattern of gene expression (RNA) gives us information on what our bodies are doing (e. g.
Transgenic Plants
Author: Leandro Peña
language: en
Publisher: Springer Science & Business Media
Release Date: 2008-02-05
The aim of Transgenic Plants: Methods and Protocols is to provide a source of information to guide the reader through a wide range of frequently used, broadly applicable, and easily reproducible techniques involved in the gene- tion of transgenic plants. Its step-by-step approach covers a series of methods for genetically transforming plant cells and tissues, and for recovering whole transgenic plants from them. The volume then moves on to the use of sele- able and reporter markers, positive selection, marker elimination after rec- ery of transgenic plants, and the analysis of transgene integration, expression, and localization in the plant genome. Although contributors usually refer to model plants in most chapters, the protocols described herein should be widely applicable to many plant species. The last two sections are devoted to me- ods of risk assessment and to exploring the current and future applications of transgenic technology in agriculture and its social implications in a case study. Transgenic Plants: Methods and Protocols is divided into six major s- tions plus an introduction, comprising 27 chapters. Part I, the Introduction, is a review of the past, present, and perspectives of the transgenic plants, from the discovery of Agrobacterium tumefaciens as a feasible transformation vector, to its use as a tool to study gene expression and function, and the current and possible future applications of this technology in agriculture, industry, and medicine.
Genetic Techniques for Biological Research
Author: Corinne A. Michels
language: en
Publisher: John Wiley & Sons
Release Date: 2002-06-10
"Genetic Techniques for Biological Research" ist ein Lehrbuch für fortgeschrittene Studenten und Doktoranden der Genetik, Molekularbiologie und Zellbiologie. Es basiert auf Fallstudien zur Hefe Saccharomyces als genetischem Modellorganismus, an dem anschaulich Theorie und Praxis der molekulargenetischen Analyse demonstriert wird. Darüber hinaus bietet es dem Leser umfassende Informationen, damit er diesen Ansatz in seine eigenen Forschungsprojekte einbauen kann. Autorin Corinne Michels - eine Expertin auf dem Gebiet der Hefegenetik und Molekularbiologie - erklärt hier genau, wie man praktische genetische Studien mit Hilfe von durchgearbeiteten Beispielen kritisch bewertet. Auf diese Weise soll der Leser die Fähigkeit zu kritischem Denken entwickeln, um das Material in eigenen Forschungsarbeiten anwenden zu können. Ein idealer Studienbegleiter zu Theorie und Praxis der molekulargenetischen Analyse!