Super Resolution Imaging Of Plasmonic Near Fields
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Plasmonics and Super-Resolution Imaging
Plasmonics is an emerging field mainly developed within the past two decades. Due to its unique capabilities to manipulate light at deep subwavelength scales, plasmonics has been commonly treated as the most important part of nanophotonics. Plasmonic-assisted optical microscopy techniques, especially super-resolution microscopy, have shown tremendous potential and attracted much attention. This book aims to collect cutting-edge studies in various optical imaging technologies with advanced performances that are enabled or enhanced by plasmonics. The basic working principles, development details, and potential future direction and perspectives are discussed. Edited by Zhaowei Liu, a prominent researcher in the field of super-resolution microscopy, this book will be an excellent reference for anyone in the field of nanophotonics, plasmonics, and optical microscopy.
Super-Resolution Imaging of Plasmonic Near-fields
Plasmonic nano-objects have shown great potential in enhancing sensing, energy transfer and computing, and there has been much e↵ort to optimize plasmonic systems and exploit their field enhancement properties. Super-resolution imaging with quantum dots (QDs) is a promising method to probe plasmonic near-fields. However, due to the strong coupling between QDs and plasmons, this technique is hindered by the formation of distorted point spread functions (PSFs) and QD mislocalizations. Chapter 4 of this dissertation investigates the coupling between QDs and 'L-shaped' gold nanostructures, and demonstrates both theoretically and experimentally that this strong coupling can induce polarization- / wavelength-dependent changes to the apparent QD emission intensity, polarization and position. From the magnitude and direction of the PSF shift under emission polarization modulation, the coupling strength can be extracted, and the true PSF location can be back-calculated from tabulated theoretical and experimental values. This discovery helps to better apply super-resolution imaging techniques to detect the plasmonic near-fields.Besides using fluorescence intensity as the local-field intensity indicator, photophysical properties of the emitter (e.g. on-time ratio) have shown to be a great candidate as well. Super-resolution fluctuation imaging (SOFI) has great potential in extracting the photophysical properties of emitters with super-resolution. In chapter 5, I discuss an open-source, modular SOFI analysis package we built for both reconstructing super-resolved plasmonic near-fields and engaging the SOFI community with a wide range of applications. Chapter 6 demonstrates how we characterize the photophysical properties of a specific fluorescent protein suitable for SOFI analysis. Our work provides a practical method with higher precision for plasmonic near-field mapping, which benefits many applications like biosensing and optical quantum computing.
Label-Free Super-Resolution Microscopy
This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.