Separation Of Cells And Subcellular Elements

Separation of Cells and Subcellular Elements

Separation of Cells and Subcellular Elements presents the chemical engineering approaches in bio-separation methods. This book evaluates the cellular aggregation in turbulent suspension, the phase partition of cells and subcellular particles, and the continuous free-flow electrophoresis. The first chapters deal with flow cytometric characterization of tumor associated changes in gynecologic malignancies; steady state rheolectrolysis; and electrophoretic approaches applicable to cell separation. The succeeding chapters consider the chromosome separation by velocity sedimentation at unit gravity and the separation of human lymphocytes based on volume and density. Other chapters describe the analysis of the gradient former for zonal rotors and the centrifugal elutriation of living cells. The last chapters are devoted to the human platelet isolation from whole blood on metrizamide gradients. The book can provide useful information to biochemists, cytologists, students, and researchers.

Separation of Cells and Subcellular Elements

Nanoseparation Using Density Gradient Ultracentrifugation

This brief introduces the classification and mechanism of density gradient ultracentrifugation (DGUC) method with rich examples showing the versatility of such an efficient separation technique. It also gives a strict mathematical description and a computational optimization model to predict the best separation parameters for a given colloidal system. The concept of “Lab in a tube” is proposed in the last chapter, which allows the size-property relationship investigation, synthetic optimization and reaction/assembly mechanism exploration etc.