Rna Based Technologies For Functional Genomics In Plants
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RNA-Based Technologies for Functional Genomics in Plants
This book offers a unique and comprehensive overview of key RNA-based technologies, as well as their development and applications for the functional genomics of plant coding and non-coding genes. It focuses on the latest as well as classical RNA-based techniques used for studies on small RNAs, long non-coding RNAs and protein-coding genes. These techniques chiefly focus on target mimics (TMs) and short tandem target mimics (STTMs) for small RNAs, and artificial microRNAs (amiRNAs), RNA interference (RNAi) and CRISPR/Cas for genes. Furthermore, the book discusses the latest trends in the field and various modifications of the above-mentioned approaches, and explores how these RNA-based technologies have been developed, applied and validated as essential technologies in plant functional genomics. RNA-based technologies, their mechanisms of action, their advantages and disadvantages, and insights into the further development and applications of these technologies in plants are discussed. These techniques will enable the users to functionally characterize genes and small RNAs through silencing, overexpression and editing. Gathering contributions by globally respected experts, the book will appeal to students, teachers and scientists in academia and industry who are interested in horticulture, genetics, pathology, entomology, physiology, molecular genetics and breeding, in vitro culture & genetic engineering, and functional genomics.
Plant Functional Genomics
Author: Erich Grotewold
language: en
Publisher: Springer Science & Business Media
Release Date: 2008-02-03
Functional genomics is a young discipline whose origin can be traced back to the late 1980s and early 1990s, when molecular tools became available to determine the cellular functions of genes. Today, functional genomics is p- ceived as the analysis, often large-scale, that bridges the structure and organi- tion of genomes and the assessment of gene function. The completion in 2000 of the genome sequence of Arabidopsis thaliana has created a number of new and exciting challenges in plant functional genomics. The immediate task for the plant biology community is to establish the functions of the approximately 25,000 genes present in this model plant. One major issue that will remain even after this formidable task is c- pleted is establishing to what degree our understanding of the genome of one model organism, such as the dicot Arabidopsis, provides insight into the or- nization and function of genes in other plants. The genome sequence of rice, completed in 2002 as a result of the synergistic interaction of the private and public sectors, promises to significantly enrich our knowledge of the general organization of plant genomes. However, the tools available to investigate gene function in rice are lagging behind those offered by other model plant systems. Approaches available to investigate gene function become even more limited for plants other than the model systems of Arabidopsis, rice, and maize.
Molecular and Biotechnological Tools for Plant Disease Management
This book aims to present the latest advances in the application of plant biotechnology and molecular biology in disease management. Among these, molecular techniques can be used to accelerate the detection and identification of pests and pathogens in plants. Also, the identification of resistance genes, characterization of desired resistance genes, and transfer of resistance gene(s) into plants have been performed by applying molecular techniques. The book will collect chapters exploring emerging areas of plant biotechnology including RNAi technology, genetic engineering, GWAS, CRISPR, and nanobiotechnology, and their applications in plant disease management and provides the latest concepts and advances in the field of plant pathology, including detection, identification, characterization, classification and diagnosis, host resistance, disease forecasting, management of plant pests and pathogens, and plant biotechnological approaches. This book offers a valuable reference for educators, students, and researchers in all disciplines of the agricultural sciences, life sciences, and biotechnology at universities, research institutions, and biotechnology companies.