Primer Design
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Primer Design
Primer Design, Volume 57 in the Methods in Microbiology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Methods on discriminating between bacterial strains, Primers to PCR and qPCR techniques, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Microbiology serials - Updated release includes the latest information on Primer Design
PCR Primer Design
Author: Anton Yuryev
language: en
Publisher: Springer Science & Business Media
Release Date: 2008-02-03
In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as transcriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome. In PCR Primer Design, authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome-scale experiments and for small-scale individual PCR amplifications. This volume will be useful for organizations performing whole genome studies, companies designing instruments that utilize PCR, and individual scientists – geneticists, molecular biologists, molecular geneticists, and more – who routinely use PCR in their research.
PCR Cloning Protocols
Author: Bing-Yuan Chen
language: en
Publisher: Springer Science & Business Media
Release Date: 2008-02-05
PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.