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Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria
Author: Frans J. de Bruijn
language: en
Publisher: John Wiley & Sons
Release Date: 2016-07-01
Bacteria in various habitats are subject to continuously changing environmental conditions, such as nutrient deprivation, heat and cold stress, UV radiation, oxidative stress, dessication, acid stress, nitrosative stress, cell envelope stress, heavy metal exposure, osmotic stress, and others. In order to survive, they have to respond to these conditions by adapting their physiology through sometimes drastic changes in gene expression. In addition they may adapt by changing their morphology, forming biofilms, fruiting bodies or spores, filaments, Viable But Not Culturable (VBNC) cells or moving away from stress compounds via chemotaxis. Changes in gene expression constitute the main component of the bacterial response to stress and environmental changes, and involve a myriad of different mechanisms, including (alternative) sigma factors, bi- or tri-component regulatory systems, small non-coding RNA’s, chaperones, CHRIS-Cas systems, DNA repair, toxin-antitoxin systems, the stringent response, efflux pumps, alarmones, and modulation of the cell envelope or membranes, to name a few. Many regulatory elements are conserved in different bacteria; however there are endless variations on the theme and novel elements of gene regulation in bacteria inhabiting particular environments are constantly being discovered. Especially in (pathogenic) bacteria colonizing the human body a plethora of bacterial responses to innate stresses such as pH, reactive nitrogen and oxygen species and antibiotic stress are being described. An attempt is made to not only cover model systems but give a broad overview of the stress-responsive regulatory systems in a variety of bacteria, including medically important bacteria, where elucidation of certain aspects of these systems could lead to treatment strategies of the pathogens. Many of the regulatory systems being uncovered are specific, but there is also considerable “cross-talk” between different circuits. Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria is a comprehensive two-volume work bringing together both review and original research articles on key topics in stress and environmental control of gene expression in bacteria. Volume One contains key overview chapters, as well as content on one/two/three component regulatory systems and stress responses, sigma factors and stress responses, small non-coding RNAs and stress responses, toxin-antitoxin systems and stress responses, stringent response to stress, responses to UV irradiation, SOS and double stranded systems repair systems and stress, adaptation to both oxidative and osmotic stress, and desiccation tolerance and drought stress. Volume Two covers heat shock responses, chaperonins and stress, cold shock responses, adaptation to acid stress, nitrosative stress, and envelope stress, as well as iron homeostasis, metal resistance, quorum sensing, chemotaxis and biofilm formation, and viable but not culturable (VBNC) cells. Covering the full breadth of current stress and environmental control of gene expression studies and expanding it towards future advances in the field, these two volumes are a one-stop reference for (non) medical molecular geneticists interested in gene regulation under stress.
Regulation of Gene Expression in Enteropathogenic Bacteria, Volume II
Following the success of this Research Topic http://journal.frontiersin.org/ researchtopic/3298/regulation-of-gene-expression-in-enteropathogenic-bacteria, we are happy to launch a second edition of the project. Pathogenic bacteria have evolved numerous strategies to survive in and to attack hosts, which can be reflected by transcriptional and posttranscriptional changes in specific genes especially including those encoding virulence determinants. Regulation of gene expression by regulatory proteins and non-coding RNAs enables the pathogens to adapt their metabolic needs and to coordinately express virulence determinants during different stages of infection.
Manual for Soil Analysis - Monitoring and Assessing Soil Bioremediation
Author: Rosa Margesin
language: en
Publisher: Springer Science & Business Media
Release Date: 2005-12-15
Reliable methods for monitoring and assessing soil quality are a prerequisite for successful soil bioremediation projects. The fifth volume of Soil Biology presents detailed descriptions of selected methods for evaluating, monitoring and assessing bioremediation treatments of soils contaminated with organic pollutants or heavy metals. Traditional soil investigation techniques, including chemical, physical and microbiological methods, are complemented by the most suitable modern methods, such as the use of bioreporter technology, immunological, ecotoxicological or molecular assays. Feasibility studies for bioremediation treatments complete the manual. Easy-to-follow protocols with step-by-step procedures, lists of the required equipment and reagents as well as notes on the evaluation and quality control allow immediate application. Short introductions to the principles and objectives help to assess the field of application of each procedure.