Parvimonas Micra

Case Reports in Infectious Diseases – Surveillance, Prevention and Treatment

The Bacterial Cell Wall

This detailed volume explores methods currently used to investigate the cell wall of various bacterial species and pathogens. By using a combination of genetic, molecular, biochemical, and cytological techniques, the protocols address many fundamental questions involving the composition, biosynthesis, and regulation of bacterial peptidoglycan. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, The Bacterial Cell Wall: Methods and Protocols provides current and future researchers with a compilation of many of the most important and useful procedures in a single resource.

Application and Reliability Assessment of Next Generation Sequencing (NGS) and targeted NGS (tNGS) in the Diagnosis of Infectious Diseases, volume III

Given the success of "Improving the Clinical Effectiveness of Metagenomic Next Generation Sequencing (mNGS) in Infection Disease Diagnosis and Treatment: Linking the NGS Specialists and Clinicians" series of article collections, and the rapidly evolving subject area, we are pleased to announce the launch of Volume III. Rapid and accurate diagnosis is crucial for the effective treatment of infectious diseases. Metagenomics, using next-generation sequencing (NGS) or targeted NGS (tNGS) by enriching and sequencing specific regions of microbial genomes, can directly analyze mixed genomic materials from bacteria, viruses, fungi, parasites, and hosts in clinical samples. This provides immense potential for cost reduction and faster turnaround times, ranging from 12 to 24 hours, without the need for a clinical hypothesis. Although NGS still faces technical and practical challenges, it has been used in clinical practice, especially for critically ill patients in intensive care units who show no response to anti-infective therapies. At this stage, it is crucial to focus on the accuracy of pathogens identification in NGS reports for downstream clinical decision-making, such as adjusting anti-infective therapies or excluding infectious diseases in cases with negative reports. Therefore, the specificity of the reported pathogens should be comprehensively assessed by combining multiple clinical tests and processes.