Multiplex Biomarker Techniques
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Multiplex Biomarker Techniques
This detailed volume explores the application of multiplex biomarker methods in the critical area of COVID-19 research through state-of-the-art technologies in the fields of genomics, proteomics, transcriptomics, metabolomics, and imaging. The book features a series of protocols from labs across the globe employing multiplex molecular approaches, which can be applied to accelerate progress in the research of SARS-CoV-2 and other infectious illnesses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and essential, Multiplex Biomarker Techniques: Methods and Applications for COVID-19 Disease Diagnosis and Risk Stratification serves as a vital resource for researchers in the areas of virology, metabolic diseases, respiratory disorders, as well as to clinical scientists, physicians, pharmacologists, and the healthcare services.
Multiplex Biomarker Techniques
This detailed book arrives as there is an increasing need for multiplex biomarker readouts for improved clinical management and to support the development of new drugs by pharmaceutical companies, due to continuous technical developments and new insights into the high complexity of many diseases. Chapters explore the basic technology platforms being applied in the fields of genomics, proteomics, transcriptomics, metabolomics, and imaging, which are currently the methods of choice in multiplex biomarker research. The book also describes the chief medical areas in which the greatest progress has been made and highlight areas where further resources are required. Written for the highly successful Methods in Molecular Biology series, methodology chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and invaluable, iMultiplex Biomarker Techniques: Methods and Applications
Laboratory Techniques in Cellular and Molecular Medicine
The methodological book ""Laboratory techniques in cellular and molecular medicine"" is intended for students of bachelor, master, and doctoral study programmes at faculties of science, medicine, and veterinary medicine, as well as for laboratory technicians interested in methodological approaches of contemporary cellular and molecular medicine. The book does not aim to provide a comprehensive overview of the current state of the art in cellular and molecular medicine, that would be a superhuman task. The aim of the 56-member author team is to provide readers with an overview of the methods established and tested at the Institute of Molecular and Translational Medicine of the Faculty of Medicine of Palacký University Olomouc – to describe the methodological principles and their practical application. It focuses both on basic methods, whose principles are used by the most modern methods, and on special methods, reflecting the laboratory experience and specialisation of individual authors. The 52 chapters describe the work with cells and microorganisms, bioengineering manipulations of nucleic acids, the search for biomarkers, detection at the level of DNA, RNA, protein, organelle, and whole cell, and the use of fluorescent and radioactive labeling. To aid comprehension, the description of the methods is illustrated by figures and diagrams. Each chapter is followed by troubleshooting tips. The book promotes the best laboratory practice to increase the reproducibility of results in biomedicine.