Mtt Assay Data Analysis
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Cancer Cell Culture
Author: Simon P. Langdon
language: en
Publisher: Springer Science & Business Media
Release Date: 2008-02-01
The culture of cancer cells is routinely practiced in many academic research centers, biotechnology companies, and hospital laboratories. Cancer Cell Culture: Methods and Protocols describes easy-to-follow methods to guide both novice and more experienced researchers seeking to use new techniques in their laboratories. Our present understanding of the cell and molecular biology of cancer has been derived mainly from the use of cultured cancer cells and we cover a number of the most widely used assays to study function in current use. Part I introduces the basic concept of cancer cell culture and this is followed by a description of the general techniques used in many cell culture facilities. The importance of cell line characterization is now widely recognized and methods to characterize and authenticate cell lines are described in Part II. Part III covers the isolation and development of specific cancer cell types and provides valuable tips for those wishing to derive new cell line models. A wide range of procedures encompassing many of the key functional features of cancer cells are described in Part IV including assays to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Methods to modify cancer cells are described in Part V, including protocols for transfection, development of drug-resistance, immortalization, and transfer in vivo. In Part VI methods of coculture of different cell types and contamination of cell lines are covered.
Basic Statistical Methods and Models for the Sciences
The use of statistics in biology, medicine, engineering, and the sciences has grown dramatically in recent years and having a basic background in the subject has become a near necessity for students and researchers in these fields. Although many introductory statistics books already exist, too often their focus leans towards theory and few help readers gain effective experience in using a standard statistical software package. Designed to be used in a first course for graduate or upper-level undergraduate students, Basic Statistical Methods and Models builds a practical foundation in the use of statistical tools and imparts a clear understanding of their underlying assumptions and limitations. Without getting bogged down in proofs and derivations, thorough discussions help readers understand why the stated methods and results are reasonable. The use of the statistical software Minitab is integrated throughout the book, giving readers valuable experience with computer simulation and problem-solving techniques. The author focuses on applications and the models appropriate to each problem while emphasizing Monte Carlo methods, the Central Limit Theorem, confidence intervals, and power functions. The text assumes that readers have some degree of maturity in mathematics, but it does not require the use of calculus. This, along with its very clear explanations, generous number of exercises, and demonstrations of the extensive uses of statistics in diverse areas applications make Basic Statistical Methods and Models highly accessible to students in a wide range of disciplines.
Advanced Cell and Molecular Techniques
Author: Karuppusamy Arunachalam
language: en
Publisher: Springer Nature
Release Date: 2025-04-23
This detailed volume explores laboratory protocols and methodologies for studying cell cultures, genetic analysis, and various biochemical assays. The chapters within this book cover a wide range of techniques, from basic cell culture methods to more specialized assays used in the study of genotoxicity, apoptosis, and gene expression, not only relevant to academic researchers but also to professionals in the pharmaceutical, biotechnological, and clinical fields. Written for the Springer Protocols Handbooks series, each protocol has been carefully compiled to provide step-by-step instructions, ensuring clarity and reproducibility. Authoritative and practical, Advanced Cell and Molecular Techniques: Protocols for In Vitro and In Vivo Studies serves as both a hands-on guide and a source of inspiration for future research as the fields of molecular biology and toxicology continue to evolve.