Methods For Monitoring Mitochondrial Functions During Pathogen Infection
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Methods for Monitoring Mitochondrial Functions During Pathogen Infection
Methods for Monitoring Mitochondrial Functions During Pathogen Infection, Volume 194 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Mitochondrial Labeling with Fluorescent Proteins for Enhanced Morphological Characterization and Visualization of Intercellular Mitochondrial Transfer, Visualizing mitochondrial electron transport chain complexes and super-complexes during infection of human macrophages with Legionella pneumophila, Quantitative assessment of mitochondrial membrane potential in macrophages in sepsis, Quantification of mitochondrial reactive oxygen species in macrophages during sepsis, and much more.Additional chapters cover Quantification of intracellular and mitochondrial ATP content in macrophages during lipopolysaccharide-induced inflammatory response, Analysis of cytosolic mtDNA release during Staphylococcus Aureus infection, Monitoring mitochondria dynamics upon infection using a tomographic approach, A screening system to determine bacterial effects on MADCAM1 expression by high endothelial venule cells, Isolation of mitochondria from Mycobacteria infected cells, Monitoring mitochondrial localization of Mycobacterial proteins, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Cell Biology series - Includes the latest information on the topic of development, characterization, and applications in CAR T Cells
Mitochondrial Dysfunction
Methods in Toxicology, Volume 2: Mitochondrial Dysfunction provides a source of methods, techniques, and experimental approaches for studying the role of abnormal mitochondrial function in cell injury. The book discusses the methods for the preparation and basic functional assessment of mitochondria from liver, kidney, muscle, and brain; the methods for assessing mitochondrial dysfunction in vivo and in intact organs; and the structural aspects of mitochondrial dysfunction are addressed. The text also describes chemical detoxification and metabolism as well as specific metabolic reactions that are especially important targets or indicators of damage. The methods for measurement of alterations in fatty acid and phospholipid metabolism and for the analysis and manipulation of oxidative injury and antioxidant systems are also considered. The book further tackles additional methods on mitochondrial energetics and transport processes; approaches for assessing impaired function of mitochondria; and genetic and developmental aspects of mitochondrial disease and toxicology. The text also looks into mitochondrial DNA synthesis, covalent binding to mitochondrial DNA, DNA repair, and mitochondrial dysfunction in the context of developing individuals and cellular differentiation. Microbiologists, toxicologists, biochemists, and molecular pharmacologists will find the book invaluable.
Molecular Detection of Human Parasitic Pathogens
Traditionally, laboratory identification of parasites has relied upon various phenotypic procedures that detect their morphological, biological, and immunological features. Because these procedures tend to be time-consuming and technically demanding, molecular methods based on nucleic acid amplification technologies have been increasingly utilized