Mbbs 2nd Year Practical Book Pdf Biochemistry

Practical Biochemistry for Colleges

This book presents a selection of tried and trusted laboratory experiments in the field of biochemistry. The experiments are described in detail and can be used directly or in a modified form. They are grouped according to a broad range of biochemical disciplines which allows those responsible for arranging practical classes to select experiments to complement any given biochemistry course. Suggestions are made for further work in more advanced classes. As well as the practical method the experiments are accompanied by background information, discussion of results, references for further study and illustrations.

Introduction to Practical Biochemistry

Competency-based Comprehensive Manual of Practical and Clinical Biochemistry

Unit 1: Introduction of Clinical Biochemistry 1. Laboratory Apparatus and Equipment, Good and Safe Laboratory Practice, and Waste Disposal Systems in Laboratory Unit 2: Qualitative Experiments and their Clinical Applications 1. Analysis of Carbohydrates 2. Analysis of Proteins 3. Analysis of Physical and Chemical Composition of Physiological Urine 4. Identify, Perform and Interpret Pathological Urine Analysis and Correlate it with Pathological States Unit 3: Quantitative Experiments and their Clinical Interpretation 1. Principle of Colorimetry 2. Principle of Spectrophotometry 3. Estimation of Blood Glucose 4. Glucose Tolerance Test and Glycated Hemoglobin 5. Liver Function Test 6. Kidney Function Test 7. Lipid Profile (Atherogenic Profile) 8. Estimation of Serum Calcium and Serum Phosphorus Unit 4: Self-Directed Learning Exercises 1. pH Meter 2. Water Homeostasis and Estimation of Na+ and K+ with ISE Analyzer 3. Arterial Blood Gas Analyzer 4. Chromatography 5. Electrophoresis 6. Enzyme-linked Immunosorbent Assay 7. Antigen-Antibody Interaction (Immunodiffusion) 8. Quality Control in Clinical Laboratory 9. DNA Isolation from Blood and Tissue Unit 5: Early Clinical Exposure Exercises and Reflective Writing 1. Analysis of Cerebrospinal Fluid 2. Thyroid Function Test 3. Pancreatic Function Tests 4. Disorders of Acid-Base Balance Unit 6: Attitude, Ethics and Communication (AETCOM) Modules 1. Introduction of Clinical Methods 2. What does it Mean to be a Doctor? 3. What does it Mean to be a Patient? 4. The Doctor-Patient Relationship 5 The Foundations of Communications Unit 7: Biochemical Calculations and Reference Range 1. Preparations of Buffers and Solutions 2. Reference Value of Various Biochemical Parameters Integration with Medicine Unit 8: Practical Spots in Biochemistry 1. Practical Spots in Biochemistry Unit 9: Competency-Based Assessment for Practical Biochemistry 1. Competency-based Assessment for Practical Biochemistry Index