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Laboratory Techniques in Cellular and Molecular Medicine

The methodological book ""Laboratory techniques in cellular and molecular medicine"" is intended for students of bachelor, master, and doctoral study programmes at faculties of science, medicine, and veterinary medicine, as well as for laboratory technicians interested in methodological approaches of contemporary cellular and molecular medicine. The book does not aim to provide a comprehensive overview of the current state of the art in cellular and molecular medicine, that would be a superhuman task. The aim of the 56-member author team is to provide readers with an overview of the methods established and tested at the Institute of Molecular and Translational Medicine of the Faculty of Medicine of Palacký University Olomouc – to describe the methodological principles and their practical application. It focuses both on basic methods, whose principles are used by the most modern methods, and on special methods, reflecting the laboratory experience and specialisation of individual authors. The 52 chapters describe the work with cells and microorganisms, bioengineering manipulations of nucleic acids, the search for biomarkers, detection at the level of DNA, RNA, protein, organelle, and whole cell, and the use of fluorescent and radioactive labeling. To aid comprehension, the description of the methods is illustrated by figures and diagrams. Each chapter is followed by troubleshooting tips. The book promotes the best laboratory practice to increase the reproducibility of results in biomedicine.

Experimental Methods in Wastewater Treatment

Over the past twenty years, the knowledge and understanding of wastewater treatment has advanced extensively and moved away from empirically based approaches to a fundamentally-based first principles approach embracing chemistry, microbiology, and physical and bioprocess engineering, often involving experimental laboratory work and techniques. Many of these experimental methods and techniques have matured to the degree that they have been accepted as reliable tools in wastewater treatment research and practice. For sector professionals, especially a new generation of young scientists and engineers entering the wastewater treatment profession, the quantity, complexity and diversity of these new developments can be overwhelming, particularly in developing countries where access to advanced level laboratory courses in wastewater treatment is not readily available. In addition, information on innovative experimental methods is scattered across scientific literature and only partially available in the form of textbooks or guidelines. This book seeks to address these deficiencies. It assembles and integrates the innovative experimental methods developed by research groups and practitioners around the world. Experimental Methods in Wastewater Treatment forms part of the internet-based curriculum in wastewater treatment at UNESCO-IHE and, as such, may also be used together with video records of experimental methods performed and narrated by the authors including guidelines on what to do and what not to do. The book is written for undergraduate and postgraduate students, researchers, laboratory staff, plant operators, consultants, and other sector professionals.