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Tears of a Hustler Pt 3
Things get serious when Marvin, the leader of a powerful upcoming gang, decides he wants to take over Pauleena's empire. With her back against the wall, Pauleena defends her territory by any means necessary even if it means killing everything moving. The streets will rain money or bloodNit's their choiceNbut giving up her throne is not an option.
The LAST FRACTION: An incredible journey into the gold fields of the Yukon
The Sawyer saga continues when Tom takes his teenage daughter and young son on an adventure that will change their lives forever. It was the Gay-Nineties for an elite few, but most of America suffered from severe economic depression. When gold was discovered in a remote part of the Yukon territories a hundred thousand desperate souls packed up and headed north to seek their fortune in the shadow of the North Pole. The Sawyer family was among them.
Directed Evolution of Selective Enzymes
Author: Manfred T. Reetz
language: en
Publisher: John Wiley & Sons
Release Date: 2016-09-07
Authored by one of the world's leading organic chemists, this authoritative reference provides an overview of basic strategies in directed evolution and introduces common gene mutagenesis, screening and selection methods. Throughout the text, emphasis is placed on methodology development to maximize efficiency, reliability and speed of the experiments and to provide guidelines for efficient protein engineering. Professor Reetz highlights the application of directed evolution experiments to address limitations in the field of enzyme selectivity, substrate scope, activity and robustness. He critically reviews recent developments and case studies, takes a look at future applications in the field of organic synthesis, and concludes with lessons learned from previous experiments.