Genetic Engineering Of Osmoregulation

Genetic Engineering of Osmoregulation

The plant world represents a vast renewable resource for production of food, chemicals and energy. The utilization of this resource is frequently limited by moisture, temperature or salt stress. The emphasis of this volume is on the molecular basis of osmoregulation, adaptation to salt and water stress and applica tions for plant improvement. A unified concept of drought, salt, thermal and other forms of stress is proposed and discussed in the publication. The volume developed from a symposium entitled "Genetic Engi neering of Osmoregulation: Impact on Plant Productivity for Food, Chemicals and Energy," organized by D. W. Rains and R. C. Valentine in cooperation with Brookhaven National Laboratory and directed by D. W. Rains and A. Hollaender. The program was supported by a grant from the National Science Founda~ion, Division of Problem Focused Research, Problem Analysis Group, and the Department of Energy. This symposium is one of several in the past and pending which deal with potential applications of genetic engineering in agri culture. Since the question was raised several times during the meeting it is perhaps a convenient time to attempt to define gene tic engineering in the context of the meeting. • Genetic engineering of osmoregulation is simply the application of the science of genetics toward osmo tically tolerant microbes and plants. • Recombinant DNA is regarded as just another tool along with conventional genetics to be utilized for improvement of microbes and plants.

Genetic Engineering of Osmoregulation Impact on Plant Productivity for Food, Chemicals, and Energy (proceedings of the Symposin N

Genetic Engineering of Plants

William C. Taylor Department of Genetics University of California Berkeley, California 94720 It is evident by now that there is a great deal of interest in exploiting the new technologies to genetically engineer new forms of plants. A purpose of this meeting is to assess the possibilities. The papers that follow are concerned with the analysis of single genes or small gene families. We will read about genes found within the nucleus, plastids, and bacteria which are responsible for agri culturally important traits. Given that these genes can be isolated by recombinant DNA techniques, there are two possible strategies for plant engineering. One involves isolating a gene from a cultivated plant, changing it in a specific way and then inserting it back into the same plant where it produces an altered gene product. An example might be changing the amino acid composition of a seed pro tein so as to make the seed a more efficient food source. A second strategy is to isolate a gene from one species and transfer it to another species where it produces a desirable feature. An example might be the transfer of a gene which encodes a more efficient pho tosynthetic enzyme from a wild relative into a cultivated species. There are three technical hurdles which must be overcome for either strategy to work. The gene of interest must be physically isolated.