Functions Of Light Microscope
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Understanding Light Microscopy
Author: Jeremy Sanderson
language: en
Publisher: John Wiley & Sons
Release Date: 2019-03-04
Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.
Micrographia
In 'Micrographia', Robert Hooke embarks on a groundbreaking exploration of the microscopic world, unveiling the previously invisible intricacies of nature through meticulous observation and detailed illustrations. This seminal work, published in 1665, represents a significant shift in scientific inquiry, paralleling the rise of the scientific revolution. Hooke's prose weaves together eloquent description with empirical observation, providing a vivid account of his experiments that range from the structure of a flea to the intricate patterns of a cork's cellular structure. His innovative use of the microscope not only revolutionizes biology but also sets a precedent for the visual representation of scientific findings. Robert Hooke, an esteemed polymath and member of the Royal Society, was deeply influenced by the intellectual currents of his time, particularly the emphasis on observation as a means of knowledge. His background in physics, architecture, and natural history equipped him with a unique perspective that allowed him to interpret his observations in innovative ways. Hooke's collaborative nature and friendship with contemporaries like Sir Isaac Newton positioned him at the forefront of scientific discourse, driving his desire to share the wonders he unearthed through his lens. '*****Micrographia'***** is indispensable for anyone seeking to understand the origins of modern microscopy and its implications on life sciences. This work not only provokes a sense of wonder about the natural world but also encourages a deeper appreciation for the intricate details that define our universe. Reading Hooke's text will enrich your understanding of both historical scientific methods and the profound nature of inquiry.
Understanding the Light Microscope
Histology, immunology, histochemistry and microscopy. Since retiring in 1989 as Reader in Anatomy at Sheffield University, he has been an independent research worker in biomedical science. Key Features * Aids insight into microscope operation and imitations * The approach is non-mathematical, yet in-depth * Enables lecture time to be replaced by learning assignments * Includes a help function for all four programs * The programs have been tried and tested by 2nd and 3rd year biomedical undergraduates.