Fluorescence Microscopy
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Fundamentals of Fluorescence Microscopy
Author: Partha Pratim Mondal
language: en
Publisher: Springer Science & Business Media
Release Date: 2013-12-12
This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
Fluorescence Microscopy
Author: F. W. D. Rost
language: en
Publisher: Cambridge University Press
Release Date: 1992
Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts, for example in living cells. This magnificent new work provides comprehensive cover of all aspects of fluorescence microscopy - including instrumentation, applications, and the history of the technique. The first volume deals with instrumentation and techniques for fluorescence microscopy, and includes a chapter on quantification and scanning. The second volume deals with the applications of fluorescence microscopy in many fields. It includes information on autofluorescence, and an invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications and suitable filter combinations.
Fluorescence Microscopy in Life Sciences
Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained. Key learning points covered in the book include: Operation of fluorescence microscopy instruments as well as the different options available today for the scientist, from the classical to the most recent approaches The wide range of biological detection possibilities that fluorescence microscopy offers in molecular biology, cell biology, histology and histopathology Fluorescent chemical compounds Breakthroughs in this field, such as non-linear microscopies and super-resolution techniques Fluorescence Microscopy In Life Sciences is intended as a detailed guide for professionals, researchers and students (including graduates Ph.D. candidates) in life sciences, with special emphasis in the biomedical field. Researchers working in allied disciplines such as pharmacology, veterinary sciences and microbiology will also benefit from the information presented in this handbook.