Flow Cytometry Basics For The Non Expert
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Flow Cytometry Basics for the Non-Expert
This first edition volume demystifies the complex topic of flow cytometry by providing detailed explanations and nearly 120 figures to help novice flow cytometry users learn and understand the bedrock principles necessary to perform basic flow cytometry experiments correctly. The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays. Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques. Easy to understand and comprehensive, Flow Cytometry Basics for the Non-Expert will be a valuable resource to novice flow cytometry users as well as experts in other biomedical research fields who need to familiarize themselves with a basic understanding of how to perform flow cytometry and interpret flow cytometry data. This book is written for both scientists and non-scientists in academia, government, biotechnology, and medicine.
Handbook of Rare Earth Elements
Author: Alfred Golloch
language: en
Publisher: Walter de Gruyter GmbH & Co KG
Release Date: 2022-07-05
The reference work describes in its new edition still more up-to-date methods for the recycling and purifi cation processes of rare earth element analysis for industrial and scientific purposes alike. Due to their vast applications, from computer hardware to mobile phones and electric cars, REEs have become a valuable resource for our modern life. New topics: emission spectroscopy, analysis of environmental samples and pharmaceutical applications.
Handbook of ELISPOT
Author: Alexander E. Kalyuzhny
language: en
Publisher: Springer Nature
Release Date: 2024-03-19
This fourth edition volume expands on the previous editions with discussions on the latest protocols that can be applied in different laboratory landscapes ranging from purely research to clinical diagnostic environments. This volume particularly focuses on ELISpot validation and evaluation of antibodies used in this assay. The chapters in this volume cover topics such as peptides in T-Cell assays and ELISpot applications for monitoring infectious diseases like tuberculosis and SARS-CoV2; FluoroSpot; the use of AI for studying ELISpot; and comparing ELISpot with flow cytometry, feline coronavirus (FCoV2), and memory b-cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Handbook of ELISPOT: Methods and Protocols, Fourth Edition is a valuable resource to aid in the advancement of science and technology, and inspire scientists and researchers to determine and pursue new discoveries in this developing field. Chapters 5, 7, 11, 13, and 15 are available open access under Creative Commons Attribution 4.0 International License via link.springer.com.