Evaluation Of Genotyping Techniques For Crytosporidium Parvum
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Evaluation of Genotyping Techniques for Crytosporidium Parvum
Author: Paul A. Rochelle
language: en
Publisher: American Water Works Association
Release Date: 2001
A research teams reports on its efforts to develop and implement DNA fingerprinting techniques to characterize and differentiate the parvus species of protozoal parasite that is ubiquitous in rivers and lakes, is relatively resistant to chlorine disinfection at commonly used concentrations, and can prove fatal for immune compromised people who become infected. They evaluate fingerprinting methods based on polymerase chain reaction, determine which method is most suitable for determining strains or isolates within a species, implement the most appropriate molecular method for determining whether the isolates can be differentiated based on its animal or human host or geographical source, and determine the particular source of the protozoa contamination in source water. The report is not indexed. c. Book News Inc.
Cryptosporidiosis and Microsporidiosis
Author: F. Petry
language: en
Publisher: Karger Medical and Scientific Publishers
Release Date: 2000-07
Cryptosporidia and microsporidia are protozoan parasites that have emerged as human pathogens over the last two decades. With the coming of AIDS they have been recognised as important opportunistic pathogens of patients with an impaired host defence system. For both groups of pathogens, chemotherapeutic intervention is extremely limited. To date, cryptosporidiosis and microsporidiosis are severe and life-threatening opportunistic infections in AIDS patients and cryptosporidiosis is a major cause of diarrhea in infants and children in developing countries. This volume provides a comprehensive overview of the biology of the pathogens as well as the diagnosis and clinical aspects of the diseases they cause. The host immune responses against Cryptosporidium and the immunology of human microsporidiosis are reviewed in detail. Further topics discussed include the epidemiology and strain variation of Cryptosporidium parvum, the epidemiology and zoonotic aspects of microsporidia, and gene discovery in Cryptosporidium. Offering a well-written and very comprehensive description of what is currently known about these emerging pathogens, this book is a valuable reference for clinical microbiologists and specialists in infectious diseases, epidemiologists in the public health sector, and physicians in general and internal medicine.
Molecular Detection of Human Parasitic Pathogens
Traditionally, laboratory identification of parasites has relied upon various phenotypic procedures that detect their morphological, biological, and immunological features. Because these procedures tend to be time-consuming and technically demanding, molecular methods based on nucleic acid amplification technologies have been increasingly utilized for rapid, sensitive, and specific characterization of parasites. The large number of original and modified molecular protocols that have been developed over the years creates a dilemma for those attempting to adopt the most appropriate protocol for streamlined identification and detection of human pathogenic organisms of interest. Part of a four-volume collection, Molecular Detection of Human Parasitic Pathogens provides a reliable and comprehensive resource on the molecular detection and identification of major human parasitic pathogens. This volume contains expert contributions from international scientists involved in human parasitic pathogen research and diagnosis. Following a similar format throughout, each chapter includes: A brief review on the classification, biology, epidemiology, clinical features, and diagnosis of an important pathogenic parasitic genus/group An outline of clinical sample collection and preparation procedures and a selection of representative stepwise molecular protocols A discussion on further research needs relating to improved diagnoses of major human parasitic pathogens This versatile reference on molecular detection and identification of major human parasitic pathogens is an indispensable tool for upcoming and experienced medical, veterinary, and industrial laboratory scientists engaged in parasite characterization. It is also suitable as a textbook for undergraduate and graduate students majoring in parasitology.