Dna Cloning A Hands On Approach
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DNA Cloning: A Hands-on Approach
This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.
A Practical Hand Book of Genes to Clones -Manual
To the student: There are a number of features to help you learn as you read. Each section is summarized with a bulleted list of key concepts. Key terms are highlighted in boldface in the text and defined in the margin for easy reference. Each chapter focuses on historical perspectives, methods, techniques and medical applications. Finally, each chapter concludes with suggested further reading, a brief list of current reviews and pivotal papers to supplement and reinforce the chapter content.
Molecular Biomethods Hand Book
Author: Ralph Rapley
language: en
Publisher: Springer Science & Business Media
Release Date: 1998-07-13
The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included.