Dissolve

Agrobacterium Protocols

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Applied Chemistry : Theory And Practice

Is An Amalgam Of Theory And Experiments. It Serves As A Laboratory Manual Of Examination, Testing, Characterisation And Evaluation Of A Few Materials Of Wide Industrial And Engineering Application. The Significance And Practical Utility Of The Various Tests And The Inferences Drawn Therefore Have Been Described In Detail. The Derivation Of The Formulas, Where-Ever Used, The Introduction, Theory And Related Discussion Are Quite Elaborate And Touch The Level Of A Theory Text.The Book Has Been Designed To Cover The Laboratory Courses In Applied Chemistry At The Various Engineering And Technical Institutions. The Book Will Be Useful To The Students Where Applied Chemistry Is Taught At The M.Sc. Level And To Public Health/Water Analysis Laboratories. It Will Also Be Useful To The Students Of Industrial Chemistry A Subject That Is Being Introduced At The Undergraduate Level In Some Of The Universities.Students Of All Levels Of Intelligence From Very Weak To Extremely Brilliant Will Find Something Of Interest To Them In The Chapter On Solutions To Viva-Voce Questions A Striking Feature Of The Book.

Procedures Used at the National Bureau of Standards to Determine Selected Trace Elements in Biological and Botanical Materials