Challenges And Solutions In Sample Preparation For High Resolution Cryo Electron Microscopy

Challenges and Solutions in Sample Preparation for High-Resolution Cryo-Electron Microscopy

Transmission Electron Microscopy Techniques

"Transmission Electron Microscopy Techniques" is a comprehensive guide that explores the use of transmission electron microscopes (TEM) to study materials at the atomic level. TEMs use electrons instead of light to magnify objects, achieving resolutions millions of times greater than light microscopes. We cover all aspects of TEM, from the basic principles of how it works to the latest advancements in the field. This book includes practical information on using a TEM and troubleshooting potential issues. Complex concepts are explained clearly and simply, making them accessible to those new to TEM. The book features many diagrams, micrographs, and schematics to help visualize the discussed concepts. We explore how TEM is used in various fields, such as materials science, biology, and nanotechnology, and discuss the latest advancements in TEM technology, including aberration-corrected microscopy and cryo-TEM. Practical guidance is provided on using a TEM and troubleshooting common problems. "Transmission Electron Microscopy Techniques" is a valuable resource for students, researchers, and professionals interested in TEM and its applications.

Cryo-electron Tomography

Cryo-Electron Tomography: A Journey from Sample Preparation to Data Mining providing a holistic overview of this rapidly advancing field and equipping researchers with the knowledge and tools necessary to initiate their own investigations. The book begins with a section on advanced cryogenic sample preparation for in situ cellular tomography, covering a range of sample types including viruses, bacteria, eukaryotic cells, and perspectives for organoids and tissues. Specific chapters focus on essential approaches such as vitrification, sample thinning, and bimodal correlative techniques. The book then transitions to a section on data mining and validation. - Offers a comprehensive overview of cryo-ET sample preparation and data mining protocols, consolidating approaches in a single resource - Includes foundational knowledge underpinning the field - Explores the latest methods, techniques and recent developments in cryo-ET - Offers guidance to researchers for key procedures - Written by an international range of experts