Antigen Processing And Presentation Protocols
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Antigen Processing and Presentation Protocols
Author: Joyce C. Solheim
language: en
Publisher: Springer Science & Business Media
Release Date: 2007-10-26
Antigen processing and presentation, as a field, explores a broad range of protein interactions and functions, both intracellular (in the cytoplasm and in the endoplasmic reticulum) and at the cell surface (between T cells and MHC molecules). To investigate such a diverse array, it is necessary that biochemical, cell biology, and immunological techniques all be employed. The purpose of Antigen Processing and Presentation Protocols is therefore to detail the most up-to-date techniques being used in this burgeoning field. Such techniques include those used to question how MHC-binding peptides are generated, to test how peptides are delivered to MHC molecules, to analyze MHC peptide-binding patterns, and to assay the T-cell response to MHC/peptide. Antigen Processing and Presentation Protocols should aid both those new and those experienced in this area of research in extending the questions that can be asked and answered by the application of these current methods. For editorial assistance, I would like to thank Angela Beninga and Rachael Turnquist.
Macrophages
Macrophages are an important part of the immune response and are characterized by their ability to phagocytose foreign matter. However the difficulties involved in macrophage isolation mean they are some of the body's least explored cells. Macrophage Methodology describes how to isolate moderate to high yields of viable cells from a variety of specific tissue sites under both normal and pathological conditions and then goes on to give protocols for macrophage purification. The third chapter covers techniques used to identify and measure endocytic and phagocytic capabilities using immunochemistry and fluorescent analysis. Chapter four identifies the key issues relating to the study of macrophages as antigen presenting cells and has protocols for the major assays used to measure antigen processing and presentation. Also covered are the theoretical and practical issues related to the processing and presentation of intracellular pathogens for which macrophages are the major host cell. The methods described for measuring macrophage secretory products concentrate on bioassays for molecules where no ELISA is available. The next two chapters cover measuring macrophage activity in vitro and in vivo. Finally methods are described for the analysis of gene expression in macrophages. A variety of broad techniques have been brought together in one affordable volume to make Macrophage Methodology an essential buy for anyone studying macrophages.
The ELISA Guidebook
Author: John R. Crowther
language: en
Publisher: Springer Science & Business Media
Release Date: 2008-02-04
John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.