Analysis Of Cytosine Methylation In Soybean Pathogen Phytophthora Sojae

ANALYSIS OF CYTOSINE METHYLATION IN SOYBEAN PATHOGEN PHYTOPHTHORA SOJAE

Phytophthora sojae is plant pathogenic oomycete that targets soybean plants and undergoes a diverse set of life stages specific to its environmental surroundings. Corresponding changes in gene expression occur, although the mechanisms by which genes are differentially expressed and silenced are not fully understood. Methods in regulation of gene expression in P. sojae may include various mechanisms at the transcriptional or post-transcriptional level, including DNA CpG methylation. Among the group of regulated genes in P. sojae are avirulence genes, which encode effector proteins that are utilized during infection. Hundreds of Avr genes are present in the P. sojae genome, although their expression is not uniform across different races. In this study, we investigate the effects of low levels of the cytosine methyltransferase inhibitor 5-aza-2’-deoxycytidine on growth and pathogenicity, in conjunction with qRT-PCR analysis of the housekeeping gene, actin, and the avirulence gene, Avr1a.

Identification and Characterization of Contrasting Genotypes/Cultivars to Discover Novel Players in Crop Responses to Abiotic/Biotic Stresses

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