Advanced Techniques In Soil Microbiology
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Advanced Techniques in Soil Microbiology
Author: Ajit Varma
language: en
Publisher: Springer Science & Business Media
Release Date: 2007-07-25
This book presents a wide range of biotechnological methods for application in soil microbiology analysis, including all essential methods involving molecular biology, immunology, microbiology, and structural biology, such as transcriptome analysis, RNAi technology, molecular matchmaking, RAPD, T-RFLP and FT/MS. The techniques and procedures presented here offer practical guides for immediate use in the laboratory. This volume will be of use both to the first-timer and to the experienced scientist.
Microbiology of Extreme Soils
Author: Patrice Dion
language: en
Publisher: Springer Science & Business Media
Release Date: 2007-12-22
My auxiliaries are the dews and rains which water this dry soil, and what fertility is in the soil itself, which for the most part is lean and effete. – Henry David Thoreau, Walden Pond The concerns that Thoreau had about his beans were nothing to those that would face a similarly conscientious gardener in the Atacama Desert or on the planet Mars, where dews are rare, or frozen, and rains are extremely rare – or absent al- gether. Yet we live in a time when an appreciation of the differences and simila- ties among soils (or regolith: no organics detected on Mars, as yet!) can provide a perspective on life at its most fundamental level: that of microbiology. Microbes are the Earth’s finest chemists, and most prodigious chemical engineers. Beyond pure chemistry, they know tricks with electrons that would make any Silicon Valley chip designer blush with pride. And yet their size and association with human food (good) and diseases (bad) has for more than a century obscured their essential place in making the Earth a habitable planet for humans. One of the most interesting facets of this book is that we are shown those chemists at work in one of their most important habitats. Soils comprise both a pervasive environment on our planet and one of the most important (even most fruitful!) of habitats with respect to human survival.
Advanced Techniques in Diagnostic Microbiology
Author: Yi-Wei Tang
language: en
Publisher: Springer Science & Business Media
Release Date: 2007-01-16
Clinical microbiologists are engaged in the field of diagnostic microbiology to determine whether pathogenic microorganisms are present in clinical specimens collected from patients with suspected infections. If microorganisms are found, these are identified and susceptibility profiles, when indicated, are determined. During the past two decades, technical advances in the field of diagnostic microbiology have made constant and enormous progress in various areas, including bacteriology, mycology, mycobacteriology, parasitology, and virology. The diagnostic capabilities of modern clinical microbiology laboratories have improved rapidly and have expanded greatly due to a technological revolution in molecular aspects of microbiology and immunology. In particular, rapid techniques for nucleic acid amplification and characterization combined with automation and user-friendly software have significantly broadened the diagnostic arsenal for the clinical microbiologist. The conventional diagnostic model for clinical microbiology has been labor-intensive and frequently required days to weeks before test results were available. Moreover, due to the complexity and length of such testing, this service was usually directed at the hospitalized patient population. The physical structure of laboratories, staffing patterns, workflow, and turnaround time all have been influenced profoundly by these technical advances. Such changes will undoubtedly continue and lead the field of diagnostic microbiology inevitably to a truly modern discipline. Advanced Techniques in Diagnostic Microbiology provides a comprehensive and up-to-date description of advanced methods that have evolved for the diagnosis of infectious diseases in the routine clinical microbiology laboratory. The book is divided into two sections. The first techniques section covers the principles and characteristics of techniques ranging from rapid antigen testing, to advanced antibody detection, to in vitro nucleicacid amplification techniques, and to nucleic acid microarray and mass spectrometry. Sufficient space is assigned to cover different nucleic acid amplification formats that are currently being used widely in the diagnostic microbiology field. Within each technique, examples are given regarding its application in the diagnostic field. Commercial product information, if available, is introduced with commentary in each chapter. If several test formats are available for a technique, objective comparisons are given to illustrate the contrasts of their advantages and disadvantages. The second applications section provides practical examples of application of these advanced techniques in several "hot" spots in the diagnostic field. A diverse team of authors presents authoritative and comprehensive information on sequence-based bacterial identification, blood and blood product screening, molecular diagnosis of sexually transmitted diseases, advances in mycobacterial diagnosis, novel and rapid emerging microorganism detection and genotyping, and future directions in the diagnostic microbiology field. We hope our readers like this technique-based approach and your feedback is highly appreciated. We want to thank the authors who devoted their time and efforts to produce their chapters. We also thank the staff at Springer Press, especially Melissa Ramondetta, who initiated the whole project. Finally, we greatly appreciate the constant encouragement of our family members through this long effort. Without their unwavering faith and full support, we would never have had the courage to commence this project.