A Practical Guide To Dna Based Methods For Biodiversity Assessment Advanced Books Https Doi Org 10 3897 Ab E68634
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A practical guide to DNA-based methods for biodiversity assessment. Advanced Books. https://doi.org/10.3897/ab.e68634
This document aims to summarise the scientific consensus relating to every step of the field and laboratory workflows involved in the most common types of samples and analyses. We do not go into great detail regarding bioinformatics (computational processing of sequence data) and data analysis since these are extensive topics in their own right. We uniquely set the field and lab steps in the context of the practical and logistical constraints faced by environmental managers in terms of cost, logistics, safety, ease-of-use, and quality assurance, highlighting key decisions to be made and the inherent trade-offs associated with the various options. We hope that this will support non-experts, and those new to the field, to navigate the key considerations associated with planning or evaluating monitoring programmes using DNA-based monitoring methods. Additionally, it will aid decision-makers in writing and evaluating tenders and proposals, ensuring that the methods used for a given project are fit-for-purpose and that results are correctly interpreted.
A Practical Guide to DNA-based Methods for Biodiversity Assessment
This publication is an output from EU COST Action DNAqua-Net (CA 15219 - Developing new genetic tools for bioassessment of aquatic ecosystems in Europe) and would not have been possible without the opportunities for international collaboration provided by the network, supported by COST (European Cooperation in Science and Technology). Therefore, our highest gratitude is due to Florian Leese and Agnès Bouchez who designed and led DNAqua-Net, and to programme managers Alex Weigand, Sarah Kückmann and Charlotte Frie who coordinated it. In addition to the authors, hundreds of researchers and practitioners from across Europe and further afield have contributed to the body of knowledge synthesised herein. DNAqua-Net workshops have served as the primary mechanism for consolidating knowledge and were particularly valuable for bringing together research scientists with regulators and end-users, which helped to emphasise the practical considerations in the implementation of DNA-based monitoring programmes. Workshops that ultimately fed into this publication were hosted in Germany (Florian Leese; University of Duisburg-Essen, 2017), Bosnia and Herzegovenia (Belma Kalamujić; University of Sarajevo, 2017), Hungary (Zoltán Csabai; University of Pécs, 2018), Austria (Michael Traugott; University of Innsbruck, 2018), Portugal (Pedro Beja; CIBIO, 2018), Italy (Stefano Fazi; Water Research Institute IRSA-CNR, 2019) and Cyprus (Marlen Vasquez; Cyprus University of Technology, 2019). The workshops highlighted what a collaborative community has emerged among researchers in this field, enabled to a large degree by programmes like DNAqua-Net as well as by a strong collective sense that our research has important real-world applications and is building a foundation for the years to come when we need every tool in the box to promote the protection and recovery of the natural world. We are particularly grateful to all those non-expert users of environmental DNA who fed back to us their experiences and challenges in engaging with these new methods and provided wider context as to the practical, logistical and financial constraints of routine monitoring (Iwan Jones, Simon Vitecek, Willie Duncan, Kerry Walsh and Martyn Kelly, to name just a few). These insights have helped to guide and shape research priorities, and we hope that this guide will prove a useful resource for these users as they begin to integrate these new technologies into the suite of tools at their disposal.
Environmental DNA
Author: Pierre Taberlet
language: en
Publisher: Oxford University Press
Release Date: 2018-02-02
Environmental DNA (eDNA) refers to DNA that can be extracted from environmental samples (such as soil, water, feces, or air) without the prior isolation of any target organism. The analysis of environmental DNA has the potential of providing high-throughput information on taxa and functional genes in a given environment, and is easily amenable to the study of both aquatic and terrestrial ecosystems. It can provide an understanding of past or present biological communities as well as their trophic relationships, and can thus offer useful insights into ecosystem functioning. There is now a rapidly-growing interest amongst biologists in applying analysis of environmental DNA to their own research. However, good practices and protocols dealing with environmental DNA are currently widely dispersed across numerous papers, with many of them presenting only preliminary results and using a diversity of methods. In this context, the principal objective of this practical handbook is to provide biologists (both students and researchers) with the scientific background necessary to assist with the understanding and implementation of best practices and analyses based on environmental DNA.