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Plant Genome Editing Technologies
This book reviews all important aspects of plant genome editing to shed new light on these genome editing technologies together in crop improvement and sustainable agriculture. The book is divided into three areas: the first of which reinterprets plant genome studies from an overview perspective, examining the safety and risk assessment of plants as well as breeding-related outcomes. The second area discusses recent advances in the understanding of crop improvement. A wide range of plant stress tolerance in relation to plant genome editing is then addressed, before turning attention to specific species approaches including rice, maize, wheat, and soybean. The entire book is devoted to an updated knowledge of plant genome editing technologies from all aspects of plant biology and agronomy as we move toward advancements in sustainable life research. The book will be highly recommended for agriculture biologists, agronomists, plant pathologists, and all related research areas.
The Wild Oryza Genomes
This book focuses on the latest genome sequencing of the 25 wild Oryza species, public and private genomic resources, and their impact on genetic improvement research. It also addresses the untapped reservoir of agronomically important traits in wild Oryza species. Rice is a model crop plant that is frequently used to address several basic questions in plant biology, yet its wild relatives offer an untapped source of agronomically important alleles that are absent in the rice gene pool. The genus Oryza is extremely diverse, as indicated by a wide range of chromosome numbers, different ploidy levels and genome sizes. After a 13-year gap from the first sequencing of rice in the 2002, the genomes of 11 wild Oryza species have now been sequenced and more will follow. These vast genomic resources are extremely useful for addressing several basic questions on the origin of the genus, evolutionary relationships between the species, domestication, and environmental adaptation, and also help to substantiate molecular breeding and pre-breeding work to introgress useful characters horizontally from wild species into cultivated rice.
CRISPR-Cas Methods
This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism. The chapter “CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.